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In which mode of plasmid replication does replication begin and end at oriV? Unidirectional theta replication...

In which mode of plasmid replication does replication begin and end at oriV?

  1. Unidirectional theta replication
  2. Bidirectional theta replication
  3. Rolling circle replication
  4. More than one but not all of the above
  5. None of the above
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Answer #1

A plasmid is a small circular double-stranded DNA present in the cytoplasm of bacteria.

Plasmid DNA replicates itself in order to form multiple copies, by conjugation, transformation and transfection methods,

It consists of the origin of replication named oriV where replication initiates. other components include a selectable marker gene and antibiotic resistance gene.

Replication takes place by 3 known methods

rolling circle replication model, unidirectional (Replication from 5' to 3' direction) and bidirectional methods(replication in both directions)

the replications start at oriV region of the plasmid but it stops at the termination sequence.

in conjugation, replication continues only till there is contact between 2 cells i.e the donor cell and the receiver cell.

thus there is no such evidence that the termination takes place at oriV thus the correct option is NONE OF THE ABOVE.

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