Question

cuagulase test

Answer 1

Coagulase Test is used to differentiate between organisms that produce Coagulase and organisms that do not produce coagulase.
Ex: Staphylococcus aureus (positive)
Staphylococcus epidermis and Staphylococcus saprophyticus (negative)

Principle:
Coagulase converts fibrinogen to fibrin thereby forming a clot.

Procedure:
Place a drop of Saline on a slide and add the bacterial culture using a loop. Add a drop of human/rabbit plasma and mix gently. If the given organism is coagulase-positive, clumping occurs within 10 seconds.