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You have received from a scientific collaborator some transgenic mice that have had the gene encoding...

You have received from a scientific collaborator some transgenic mice that have had the gene encoding the BRCA1 gene knocked out. Which method would help you to confirm the genotype of these mice most directly?

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Some transgenic mice that have had the gene encoding the BRCA1 gene knocked out. Southern blotting method help you to confirm the genotype of these mice most directly. Southern blotting and polymerase chain reaction i.e- PCR always confirm targetting gene knocked out so that genotype of the mice confirm most directly.

Southern blot and PCR confirm the novel amplications in BRCA1 . From a scientific collaborators a genomic DNA from case, suspected carrier of an amplification of exons and two controls were digested using BamH1 and after blotting hybridised using a RT-PCR probe containing exoms. The autoradiogram shows novel fragment of 7kb. The size of the amplified sequences as determined by sequencing is comparable with the size of the extra bands observed after Southern blot analysis, which indicates that the amplified sequences are amplified in tendem.

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