Question

If I put two primers which can amplify plasmid and do PCR, and did gel electrophoresis....

If I put two primers which can amplify plasmid and do PCR, and did gel electrophoresis.
If I do gel electrophoresis also with plasmid before PCR , what would be the result compare to the result after PCR. give me the reason and also articles which is related if it is available

Homework Answers

Answer #1

The plasmid DNA can take three different conformations including supercoiled, open-circular and linear forms. Therefore in the gel, the plasmid DNA may appear as three different bands. The super coiled conformation is much compact in size than the other forms and therefore it will have less sterric hindrance while moving in the gel. So, the supercoiled form of plasmid will move faster followed by circular and linear forms.

On the other hand, the plasmid will be there only in the linear form after the PCR amplification. Therefore the PCR amplified plasmid will be seen as a single band in the gel. Also, the intensity of the band for the PCR amplified plasmid will be higher as compared to the plasmid sample before PCR amplification.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection...
1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is the _____ blot. Select one: a. Northern b. Western c. Northeastern d. Southern e. Southwestern 2. DNA sequencing by controlled termination of replication is called the _____ method. Select one: a. Sanger b. E. coli c. Restriction enzymes d. DNA Microarray e. Ligase f. Polymerase Chain Reaction g. Footprint h. Reverse Trancriptase i. Vector j. Expression k. Fluorescent l. cDNA 3. How...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use gel electrophoresis to compare the size of 2 dye molecules Methyl orange and Ponceau G. You will also analyze an “unknown” sample that contains a mixture of two dyes. Dye molecules with lower molecular weight or greater electrical charge will migrate faster through the gel, than dye molecules with greater molecular weights or lesser electrical charge. Materials: Mini gel electrophoresis chamber 6 Tooth comb...
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember...
Procedure I. Reverse Transcription. For each of your RNA samples set up the following reaction: *Remember to use proper RNA handling technique! 1. In a 0.2 ml size RNAse-Free tube add the following: • 0.5 µg RNA (no more than 6 µl) You can add as little as 0.1 µg of RNA if your concentration is low • 2 µl dT primer • Nuclease Free Water to final volume of 8 µl * Before you start write the exact volume...
can you please do this lab? use lunix or C program its a continuation of a...
can you please do this lab? use lunix or C program its a continuation of a previous lab. the previous lab: Unix lab 4: compile and link multiple c or c++ files Please do the following tasks step by step: create a new directory named by inlab4 enter directory inlab4 create a new file named by reverse.c with the following contents and then close the file: /*reverse.c */ #include <stdio.h> reverse(char *before, char *after); main() {       char str[100];    /*Buffer...
What do you think of eac of these ideas? Do you agree or disagree for each...
What do you think of eac of these ideas? Do you agree or disagree for each one give the answer in full sentences 1. I do find his approach of deductive reasoning as a replacement of inductive reasoning a valid way to define scientific practice because the process is the process no matter if one is proving something right or wrong. I feel it can be applied to all branches and should be because as states in the reading it...
Please submit an original 50 word response to the summary below: The organization in which I...
Please submit an original 50 word response to the summary below: The organization in which I work is not fully designed to handle these types of processes but they are slowing progressing toward a more technological system. While the company does have computers, some of their systems are still outdated and need to be upgraded. An example is the system we used to clock in for work or clock out. When I started at this company about seven years ago,...
I did already posted this question before, I did get the answer but i am not...
I did already posted this question before, I did get the answer but i am not satisfied with the answer i did the code as a solution not the description as my solution, so i am reposting this question again. Please send me the code as my solution not the description In this project, build a simple Unix shell. The shell is the heart of the command-line interface, and thus is central to the Unix/C programming environment. Mastering use of...
Suppose someone has told you that he will pay you $500 if you can roll a...
Suppose someone has told you that he will pay you $500 if you can roll a die and have it land on a 6. You both agree that you will use a virtual die that can be found at this website. Be sure to set it on one die, and not two. You are not certain that using a die such as this virtual die is actually as fair as a normal plastic die and want to justify for yourself...
How do you relate the most to this story and why? In two strong paragraphs, explain...
How do you relate the most to this story and why? In two strong paragraphs, explain what it is about the tone and/or imagery of the story that speaks to you and why. Use some text examples to support your response Hills Like White Elephants (1927) The hills across the valley of the Ebro1 were long and white. On this side there was no shade and no trees and the station was between two lines of rails in the sun....
What would your response be to these two discussion board responses from students: 1. My definition...
What would your response be to these two discussion board responses from students: 1. My definition of knowledge is information that you acquire throughout your life that you can use for your benefit. Truth when talking about information is tricky because you need to do research in order to find out if something is truthful. that is where belief comes in to play. will you believe something to be true just because you saw a video on it? will you...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT