Question

1). What were the justifications given for funding the Human Genome Project?

A). Scientific discoveries

B). Facilitate technological advances

C). Understanding and treating human diseases

D). Economic impact

E). All of the above.

2). What are some of the concerns with using transcriptional Fusion reporter gene constructs to following the expression of the gene of Interest?

A ). The reporter gene fused to the gene of Interest may interfere with the proper folding and function of protein product of the gene of Interest.

B). The insertion of the transcriptional fusion construct to another part of the chromosome (heterochromatic region ) may disrupt the expression of the reporter gene.

C). The RNA polymerase complex may not recognize the promoter fused to the reporter gene.

D). The fusing the reporter gene to the gene of interest might shift the reading frame.

E). Three of the above.

F). All of the above

3 ). Reverse genetics of approaches require researchers to mutate gene or genes of Interest . Which of the following molecular technique is commonly used to identify the genes that are mutated in mutants from either insertional ( transposons ) mutagenesis or chemical mutagenesis experiments?

A ). Restriction fragment length polymorphisms for fingerprinting the mutant versus wildtype.

B). Introduction of the CRISPR/Cas9 system into the cell.

C). PCR using gene and insertion sequence - specific primers or followed by cleavage with the CIeI endonulease.

D). Introduction of dsRNA to induce RNA interference.

E). Selection for the mutants in neomycin and ganciclovir.

4). In the process of producing a gene knockout mutant in mice, which of the following responses to positive and negative selection would be consistent with No Recombination / Insertion of the knockout DNA fragment with the chromosomes within the embryonic stem cells?

A ). Neomycin sensitive, ganciclovir sensitive.

B). Neomycin sensitive, ganciclovir resistant.

C ). Neomycin resistant, ganciclovir sensitive.

D ). Neomycin resistant, ganciclovir resistant.

E ). Two of the above.

5 ). The CRISPR / Cas9 system can be engineered to do which of the following?

A ). Cut DNA at a specific location, resulting in mutations in that location during the repair process.

B). Cut DNA at a specific location, allowing for homology based repairs to be made with a Donor DNA which contains homologous sequences and the desired sequence changes.

C ). Deliver Cas9 fused with an activator protein to a specific promoter and activate the transcription of the corresponding gene.

D ). Deliver Cas9 fused with a repressor protein to a specific promoter and repress the transcription of the corresponding gene.

E ). Three of the above.

F). All of the above.

Answer 1

Please find the answers below:

Answer 1: Choice E (Human genome project was one of the major breakthroughs in scientific advanvements owing to its quality of information and economical process)

Answer 2: Choice F (All of these statements are of concern with respect to fusion proteins, hence their genetic material and localization in the euchromatin is closely observed)

Answer 3: Choice E (A mutant organism/cell is produced by insertion/modification of a gene of interest while adding a selectable marker into it, generally an antibiotic resistance gene)

Answer 4: Choice E (Neomycin resistance gene is generally inserted into the recombinants. However, ganciclovir can be used to detect a positive or negative selection based upon the nature of genetic manipulation)