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BIOCHEMISTRY 9) Reactions that replenish intermediates in the TCA cycle are called anaplerotic reactions. a) Which...

BIOCHEMISTRY

9) Reactions that replenish intermediates in the TCA cycle are called anaplerotic reactions.

a) Which of reactions in the mitochondria are anaplerotic ?

b) What are the sources of the intermediates involved in the anaplerotic reactions

10) Fluoroacetate is a potent inhibitor of the TCA cycle. Which step of the TCA cycle is inhibited as a result of fluoroacetate entering the TCA cycle

11) What are allosteric activators and inhibitors for isocitrate dehydrogenase?

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Answer #1

Ans- ANAPLEROTIC REACTIONS

1. Conversion of pyruvate to oxaloaceate.

pyruvate + CO2 + ATP oxaloacetate

This reaction is catalysed by pyruvate carboxylase.

2. Conversion of phosphoenolpyruvate to oxaloacetate.

Phosphoenolpyruvate+HCO3(−) oxaloacetate

This reaction is catalysed by phosphoenolpyruvate carboxylase.

b. The source of pyruvate and phosphoenolpyruvate is glycolysis.

2. Floroacetate inhibits enzyme aconitase that catalyses isomerization of citrate to isocitrate. The floroacetate gets converted to florocitrate.

3. The enzyme isocitrate dehydrogenase is allosterically activated by ADP and inhibited by NADH.

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