Question

15) You are observing a set of endosomes using two fluorescently molecules: GFP fused to a receptor that resides on the plasma membrane and a red fluorescently tagged cholesterol droplets that you dumped into the extracellular fluid. You notice that the endosomes are a mix of three colors. One set is yellow (contains red and green) one set is green and one set is red. Identify each set.

Answer 1

There are two florescent tags that initially used on the experiment namely GFP and the red florescent tag, GFP binds to the receptors and red tag binds to the cholesterol droplet. Here three out comes observed one is green coloured endosomes indicate the endosomes with receptors on their membrane. Red florescent endosome indicates the endoscope with cholesterol on its lipid layer and the yellow coloured endosome is indicates the endoscope which has both the receptor and the cholesterol as well.