You have a sample that includes equimolar amounts of protein, dsDNA, ssDNA and typical eukaryotic mRNA. You want to separate the 4 components of the mixture in 3-4 steps, without destroying a ny of the molecules of your sample. At the end of your separation you must have one and only chemical species in each tube (one tube with dsDNA, one with ssRNA, etc.)How do you separate a m mixture of triglycerides from some DNA? At the end of your separation you must have one and only chemical species in each tube, and your chemicals have to be intact.
4 components to be separated from each others..
To separate nucleic acid :- blotting technique is used ...
It is technique to immobize the nucleic acid like DNA / RNA n protein on nitrocellulose paper.
Southern blotting FOR DNA
Northern blotting FOR RNA
Western blotting FOR PROTEIN
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