Question

The equilibirum constant (k) for an amino acid acting as an acid-base pair in solution is:...

The equilibirum constant (k) for an amino acid acting as an acid-base pair in solution is:

AA + H2O <--------> H3O+ + AA- k = 9.03 E -5

Calculate the absorbance at 433 nm for the solution if the initial AA concentration of 4E-4 M, given that ɛAA = 9.04 E3 M and ɛAA- = 0.855 E3 M

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Answer #1

Here, absorbance   = e * L * c

where, e = molar extinction coefficient

            L = path length of the cell holder

            c = concentration of the solution

=>   absorbance   =   9.03 * 10-5 * 433 * 10-9 * 4 * 10-4

                            = 1.564 * 10-14

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