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HPLC analysis of basic analytes performs better at low pH, where they are fully ionized (2...

HPLC analysis of basic analytes performs better at low pH, where they are fully ionized (2 pH units lower than any other analyte pKa). For an analyte with pKa = 5.6 and 60%/40% MeCN/H2O eluent composition, what should the pH of an aqueous portion of the mobile phase be to ensure complete analyte ionization.

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