What is the advantage of using an internal standard in the gas chromatography - mass spectrometry experiment? (Objective was to determine the amount of n-undecane and n-dodecane using the total ion count method with n-decane as the internal standard).
In order to set the instrument right, it is important to calibrate the instrument first. This will be done by injecting a known compound with known concentration. This would enable us to ensure the mass and the quantity. In case if the instrument is slightly wrong, then it can be fixed to exact mass value and integration curve so that it would give the proper value that specifies the amount.
Doing the above mentioned process, the instrument can be fixed in case, if there exists any problem of calibration
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