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Convert in micromoles, the (change in absorbance/minute) using Beer's Law and extinction coefficient for dopachrome (3600...

Convert in micromoles, the (change in absorbance/minute) using Beer's Law and extinction coefficient for dopachrome (3600 M-1 cm-1).

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Answer #1

Accordinf to Beer's law: A = cl

A = absorbance, = extinction coefficient = 3600M-1cm-1, c= concentration and l= length of the cuvette= 1cm (usually)

Calculating concentration, c = A/l = (A/3600) M

Put the value of Absorbance and find the concentration, c.

Usually the concnetration c will be in mM, for this case say x mM.   Now, the concentration is x mM i.e. x mol in 1ml of the solution.

The concnetration you get in mM will be equivalent to the change in absorbance in micromoles.

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