Calculate the molar concentration of a solution that has a measured absorbance of 0.635 using a 1-cm pathlength cuvette and an extinction coefficient of 46,000.0 M-1cm-1.
Molar concentration from absorbance
To solve this problem, we use Beer's Law:
A= the absorbance
ε= the molar absorptivity constant
l= the path length
c= the molar concentration of the solution.
We can rearrange this equation to get
A=absorbance = 0.635
l= the path length = 1 cm
= extinction coefficient = 46,000.0 M-1 cm-1 = 46,000.0 L mol -1 cm-1
c= 0.635/ 46000 1 = 1.3810-5 mol L-1 = 1.3810-5 M
|1.3810-5 mol L-1 = 1.3810-5 M|
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