Question

So this is a practice problem for Biochem 1 and will be a huge part of an upcoming exam. I need to be sure I have all of this down and correct. I have worked on this and by the last step have gotten 3 different activation of energies and I can't figure what I'm doing wrong PLEAE HELP

1.) Complete all graphs and show work:

Mock Enzyme Activity Measurements
T = 25C
[E]total = 2 x 10-6 M
[S] mM	Vo (mM/4 sec)	Vo (mM/s)
5	6.67	1.67
10	10	2.50
15	14	3.50
20	14	3.50
25	15	3.75
30	16	4.00

A.)Plot Vo (mM/sec) versus [S]

B.) Plot 1/Vo versus 1/[S]

C.) From B above, determine Km and Vmax

D.) From the Vmax, determine kcat

E.) Determine the catalytic efficiency (kcat/Km)

F.) From the kcat, determine the activation energy

Answer 1