Question

If you increase the content of acetone in the eluent mixture (mixture of acetone/hexane), what would you expect to happen to Rf values of chlorophyll? Why? (for discussion purposes, assume chlorophyll to arrive at Rf of 0.5 under the “standard” eluent mixture.)

Answer 1

1) chlorophyll arrive at Rf 0.5 under the" standard " eluent mixture.if we increase the contents of acetone in the eluent mixture ( mixture of acetone and hexane) polarity of eluent increase.

2) Then the chlorophyll moved up the polar silica gel without being absorbed in the stationary phase . Causing the Rf value increases than standard Rf value of chlorophyll.

3)with the addition of acetone , Rf value increases which proveing that chlorophyll was a polar and could move up stationary phase due to polar solvent .