Question

Describe 3 different ways of making a buffer solution by answering the following question in 3...

Describe 3 different ways of making a buffer solution by answering the following question in 3 different ways: How could you make 350 mL of 0.200 M HEPES buffer with a pH of 7.1, if you are given HEPES acid (solid), HEPES base (sodium salt, solid) 6.0 M NaOH, and 6.0 M HCl?

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Homework Answers

Answer #1

Formulae:

Kn = Kw / Kb = 1 10 -14 / 1.75 10 -15

(OH) = 0.1 7.55 10-5

  = 7.55 106

SOLID   = 350 0.200

= 6.8799

= 7.1 6.0 / 58

= log 7.55

Base:

Ph = 5.12

POH    = 14 - Ph

= 14 - 5.12

pOH = 8.88

- log ( OH ) - = 8.88

(OH)- = 1.3 10 -9

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