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My Post Lab questions are asking me this about my caffeine experiment. "You perform a TLC...

My Post Lab questions are asking me this about my caffeine experiment. "You perform a TLC experiment on a product that you obtained in the lab. When you put it under the UV lamp, you see only one spot. Upon further analysis, however, you realize that there are actually two compounds in the sample. Give two reasons why this could happen, and then suggest how you could modify the experiment to overcome each one"

I do not know why this could happen or how to determine this.

Thank you!

Science   Chemistry   
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Answer #1

(First understand all compunds are not uv active )

so, you run more TLC under different different eluent system low polarity to high polarity ratio .

then uv active spote remark by pencil then this TLC deep in slightyl acidic and diluted KMnO4 fresh solutions for 1 sec dry it and see you will get two spots or different side spots this is second spots.

(Precaution:

1)TLC must dry moisture free before spoting

2)after spoting TLC angain dry it and then run under moisture free eluent.)

this is practical method you will get idea in more practice.

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