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In a spectrophotometer of 550 nm, and quantitate protein by using biruet reagent. Calculate true absorbance...

In a spectrophotometer of 550 nm, and quantitate protein by using biruet reagent. Calculate true absorbance of the sample

Sample

Transmittance

biuret reagent

99.42 %

biuret reagent + protein

16.59 %
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