Question

1. what method would you use to analyse 50ng of a pure protein in solution

A. biuret

B BCA

C fluorescamine

D OPA

E Lowry

F FQ or CBQ

G Bradford

H Absorbance at 280 nm

I Absorbance at 205 nm

2. what analytical method would you choose to detect and quantitate HIV antibodies in human blood

A pace method

B dumas method

C scopes method

D biuret method

E ELISA

F flow cytometry

G FRET

Answer 1

Q1)Absorbance at 280 nm

this method is recomended for calibrating bovine serum albumin or ther pure protein solution for use as standarads in other methods.becauses proteins in solution absorb UV light absorbence maxima at 280 &200nm . ammino acids with aromatic rings are the primary reason for absorbence peak at 280nm.

Q2) ELISA

it means " the enzyme -linked immunosorbent assay (ELISA) is a technique used to detect antibodies or infectious agents in a sample. Antibodies are made in responce to infection and so an antibody ELISA can indicate wether or not humans has in contact with certain virus"