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What is the utility of Beer's law and light absorbance near 280 nm in protein studies?...

What is the utility of Beer's law and light absorbance near 280 nm in protein studies?

A) determining the size of proteins

B) determining protein purity

C) estimating protein concentration

D) estimating the amount of DNA contaminant

E) none of the above

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Answer #1

Ans) The correct answer is "C" i.e estimating protein concentration

(The proteins absorb at 280nm mainly by the aromatic amino acids like tryptophan,tyrosine and phenylalanine.When the absorbance is measured by the spectrophotometer,the OD values on the Y-axis of the standard graph can be extrapolated on the X-axis to determine the protein concentration of unknown protein sample.

Option A is totally incorrect because for the size determination, SDS PAGE technique can be employed.

Options B and D are incorrect because for their measurement we calculate 260/280 nm ratio..

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