4.)Describe what your TLC results tell you about the identity of the amino acid product. Is there any evidence for the presence of any other compounds present in the reaction mixture? 5. Draw the mechanism for the acid promoted hydrolysis of the imine in the last step of Figure 3.
4) the tlc of the amino acid formed here would be more polar and would move much slowly on the tlc plate. On the other hand the PLP formed is less plar and would move faster on the plate. the Rf value which is distance travelled by solute over the dolvent would be thus greater for the PLP and lower for the amino acid product formed.
5) the mechanism of hydrolysis of the aldimine product formed in between to the free amino acid is shown below.
the mechanism is shown with a general aldimine starying material.
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