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Upon completing column chromatography and thin layer chromatography, what are the signs of good seperation? What...

Upon completing column chromatography and thin layer chromatography, what are the signs of good seperation? What are some errors that can occur to lead to a bad separation?

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Answer #1

Column and thin layer chromatography (TLC) are used to separate pure components from the crude mixture.

In TLC technique, upon elution of TLC plate spotted with the crude reaction mixture in mobile phase (solvents with different polarity), spots that are separated by a long distance on TLC plate indicates the good separation. If the crude mixture spots are in close proximity after elution, then that is said to be a bad separation in TLC.

In column chromatography, the solvent fractions that are collected from eluting the column have to be evaporated and the purity of these fractions can be checked by TLC technique. On TLC plate if the solvent fractions show only one spot then the separation in the column chromatography is said to be good. If the spots on the TLC is more than one then the separation in the column chromatography is said to be bad.

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