Question

Calculate the melting temperature for a 28.00 amino acid polyhistidine peptide at physiological pH in a 310-helix.
need numerical value using delta g equation

Answer 1

Changes in CD as a function of temperature at particular wavelengths and can be used to determine the thermodynamics of unfolding of protein or polypeptide  i.e., the van't Hoff enthalpy (ΔH) and entropy (ΔS) of unfolding, the midpoint of the unfolding transition (TM) and the free energy (ΔG) of folding. Analysis of CD spectra obtained as a function of temperature becomes also useful in predicting whether a protein has unfolding or folding intermediates.

For a polyhistidine peptide at physiological pH in a 310-helix 28.00 residues in length, the T_m= higher than 40°C.

Melting temperature of a 28.00 amino acid polyhistidine peptide at physiological pH is T_m= higher than 40°C.