Question

Calculate the melting temperature of a 28.00 amino acid α-helical peptide if that peptide contains all alanines and a single internal glycine residue. 28 is the number of amino acids

need numerical value using delta g equation

Answer 1

Changes in CD as a function of temperature at particular wavelengths and can be used to determine the thermodynamics of unfolding of protein or polypeptide  i.e., the van't Hoff enthalpy (ΔH) and entropy (ΔS) of unfolding, the midpoint of the unfolding transition (TM) and the free energy (ΔG) of folding. Analysis of CD spectra obtained as a function of temperature becomes also useful in predicting whether a protein has unfolding or folding intermediates.

For an α-helical polyalanine peptide 28.00 residues in length the T_m= higher than 40°C

Melting temperature of a 28.00 amino acid α-helical peptide if that peptide contains all alanines and a single internal glycine residue T_m= higher than 40°C.