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How does EcoRV differentiate between cognate and non-specific DNA? a. binding energy for cognate DNA is...

How does EcoRV differentiate between cognate and non-specific DNA?

a. binding energy for cognate DNA is higher than for non-specific DNA

b. magnesium is only bound in when EcoRv binds to non-specific DNA

c. DNA is distorted when EcoRV binds to the cognate sequence.

d. all of the above e. none of the above

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Answer #1

Apart from EcoRv it is restriction endonucleases which can distinguish between cognate and nonspecific DNA sequences but EcoRv also used to distingusih betweem these two. From the various group of researcher it was confirmed fro mte x-ray structure that the both congate and non specific DNA complex found diiferent in the absence of metal co factor indicating that the EcoRV specific and nonspecific binding free energies should differ substantially. So it was due to reason (a) that binding energy for congate DNA is higher than for non-specific

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