Formation of a replication fork results in:
the replication fork is formed when double-stranded DNA melts and DNA synthesis occurs in both directions, that is in a replication bubble two replication forks are formed.
DNA synthesis is continuous in the leading strand and in the lagging strand DNA synthesis is discontinuous, DNA is synthesized as fragments.
SSB proteins bind to the single-stranded DNA formed during replication.
DNA synthesis occurs in 5` to 3` direction, and no new nucleotides are added to template DNA, new nucleotides are added complementary to the template strand.
Due to the unwinding of Double-stranded DNA, supercoiling occurs in the parental DNA ahead of the replication bubble.
B) super coiling in the parental DNA ahead of the fork.
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