Question

1.How a solvent is selected as eluent in chromatography?

1.How a solvent is selected as eluent in chromatography?

Homework Answers

Answer #1

If your compound is more polar like amino acids, carboxylic acids, amines, alcohols then a more polar solvent like methanol, ethylacetate sometimes very rare cases even water is also used as an eluent in chromatography.

If your compound is not that much polar use of hexane is recommended. But we use mixture of polar and non-polar sovents used how they will work!.

I am going to explain it by taking an example of two pigmnets seperation. Please save the below image and zoom it for better clarity and resolution.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
4. How is the eluent polarity changing when the solvent is switched from 100% hexanes to...
4. How is the eluent polarity changing when the solvent is switched from 100% hexanes to 1:1 hexanes : ethyl acetate? What is the purpose of this switch?
How would you separate a mixture of benzhydrol, benzophenone, and biphenyl using column chromatography (i.e. which...
How would you separate a mixture of benzhydrol, benzophenone, and biphenyl using column chromatography (i.e. which is the best eluent or mixture of eluent, order of addition of the eluents, order of elution, ect.)?
A student using dichloromethane as the solvent runs a chromatography column and elutes with a 10:1...
A student using dichloromethane as the solvent runs a chromatography column and elutes with a 10:1 ratio of hexane/ethyl acetate. The student found that all the mixture came off the column at nearly the same time. What mistake did the student make in running the column?
Can hexane be used as a solvent to separate naphthalene and biphenyl by column chromatography with...
Can hexane be used as a solvent to separate naphthalene and biphenyl by column chromatography with silica gel as the stationary phase? why or why not?
Could chromatography be used to separate carbon from chlorine in CCl4?(carbon tetrachloride - a carcinogenic solvent)
Could chromatography be used to separate carbon from chlorine in CCl4?(carbon tetrachloride - a carcinogenic solvent)
There are two major components in the chromatography experiment: the solvent system (mobile phase) and the...
There are two major components in the chromatography experiment: the solvent system (mobile phase) and the paper (stationary phase). Which of these major components is more polar phase and which is less polar phase? Explain using Lewis structures!!
Thin Layer Chromatography: If a TLC plate is developed until the solvent front reachers the top...
Thin Layer Chromatography: If a TLC plate is developed until the solvent front reachers the top of the plate, can it be used to determine an Rf value? Please Explain
A nonpolar aromatic compounds were separated by high performance liquid chromatography on an C18 column. If...
A nonpolar aromatic compounds were separated by high performance liquid chromatography on an C18 column. If eluent solution was 50% MeOH in water,  how would the retention times be affected if 90% MeOH in water? ... Octanoic acid and 1-aminooctane were passed through the same column using an eluent of 20% MeOH/80% buffer (pH3.0). Which compound is expected to be eluted first and why? Thanks
When performing a thin layer chromatography procedure, the solvent front moved 10.0 centimeters. The substance of...
When performing a thin layer chromatography procedure, the solvent front moved 10.0 centimeters. The substance of interest moved 4.0 centimeters. What is the Rf for the substance of interest?
a) In column chromatography, does the outcome of a separation depend on the solvent that is...
a) In column chromatography, does the outcome of a separation depend on the solvent that is used to dissolve the compound mixture before applying it to the stationary phase? Please explain. b) If you perform two column chromatographic separations in two columns of equal size, one column packed with a silica gel of smaller particle size than the other, would the outcome of the separation be different? Please explain.
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT