A nonpolar aromatic compounds were separated by high performance liquid chromatography on an C18 column. If eluent solution was 50% MeOH in water, how would the retention times be affected if 90% MeOH in water? ... Octanoic acid and 1-aminooctane were passed through the same column using an eluent of 20% MeOH/80% buffer (pH3.0). Which compound is expected to be eluted first and why? Thanks
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