Question

How does spectral bandpass affect the shape and measured absorbance of molecular absorptions?

How does spectral bandpass affect the shape and measured absorbance of molecular absorptions?

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Answer #1

Spectral Band Width (SBW) is also called as spectral bandpass. It is used to measure the quality of light from monochromator. Generally larger the spectral bandpass results smaller the quality, which in turn decreases the accuracy of absorbance measurements also. Increasing band width decreases absorption and also peak shape distorts to collapse into a broader peak. Please see below image for better understanding.

You may also consider solvent can affect position and shape of curve. Polar solvents broaden out peaks, eliminates fine structure.

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