Question

In column chromatography, one the chromatographic column has been packed, why is it important to drain...

In column chromatography, one the chromatographic column has been packed, why is it important to drain the level of the liquid in the column to just above the top of the column of silica gel before applying the compound to be separated?

Homework Answers

Answer #1

This is required to avoid the solubility of compound (which is to be seperated) through out whole solvent which is present excess in the column. If solvent is present in excess, then there are chances that mixture of compounds will be distrributed in the excess solvent present in column due to some solubility and due to this issue, compund will start comming covering a broader region in silicagel instead of a narrow band which is required for better separation.

Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Column chromotography lab question? Once the chromatographic column has been prepared, why is it important to...
Column chromotography lab question? Once the chromatographic column has been prepared, why is it important to allow the level of the liquid in the column to drop to the level of the alumina before applying the solution of the compound to be separated?
a) In column chromatography, does the outcome of a separation depend on the solvent that is...
a) In column chromatography, does the outcome of a separation depend on the solvent that is used to dissolve the compound mixture before applying it to the stationary phase? Please explain. b) If you perform two column chromatographic separations in two columns of equal size, one column packed with a silica gel of smaller particle size than the other, would the outcome of the separation be different? Please explain.
In column chromatography, why is it important not to let the level of the eluding to...
In column chromatography, why is it important not to let the level of the eluding to drop below the top of the absorbent column?
Why has medicine been important to the overall development of technology? please answer with at least...
Why has medicine been important to the overall development of technology? please answer with at least one page (250-300 words) thank you!
The environment is one of many important correlates of health outcomes. Pollution has been a major...
The environment is one of many important correlates of health outcomes. Pollution has been a major public policy issue in the United States, mainly due to the negative externalities on health associated with a polluted environment. Power production produces large amounts of air pollution. Assume XYZ operates a power plant in the local market. XYZ’s marginal cost function is: MC(q) = 2+0.1q. XYZ can sell all the output he produces for $9 per unit. In generating power, XYZ also emits...
Executive Pay: Make the Link One of the most important issues in organizations recently has been...
Executive Pay: Make the Link One of the most important issues in organizations recently has been that of executive pay. As we have already seen, executive pay is usually quite high. This activity has to do with the way it is determined. This case focuses on the chairman of the board, Mary Cohn, at Super Mega Corp, a Fortune 100 company that is looking for a new CEO. As she prepares to negotiate a pay package with the board's chosen...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use...
Lab 9 – Molecular Biology In this lab, you will prepare an agarose gel and use gel electrophoresis to compare the size of 2 dye molecules Methyl orange and Ponceau G. You will also analyze an “unknown” sample that contains a mixture of two dyes. Dye molecules with lower molecular weight or greater electrical charge will migrate faster through the gel, than dye molecules with greater molecular weights or lesser electrical charge. Materials: Mini gel electrophoresis chamber 6 Tooth comb...
A manufacturing company has been selected to assemble a small but important component that will be...
A manufacturing company has been selected to assemble a small but important component that will be used during the construction of numerous infrastructure projects. The company anticipates the need to assemble several million components over the next several years. Company engineers select three potential assembly methods: Method A, Method B, and Method C. Management would like to select the method that produces the fewest number parts per 10,000 parts produced that do not meet specifications. It may also be possible...
One area of concern for the accounting profession for the past 20 years has been the...
One area of concern for the accounting profession for the past 20 years has been the proliferation of alternative practice structures. Potential problems exist because the audit side of the business may be influenced by the public entity that controls it. One such situation involves K&B, CPA Associates, and Cryden Business and Tax Services. Billy Kamen, CPA, has been a partner of K&B for more than 30 years. He thought he had seen it all in the accounting profession. The...
The Republican tax cut has been in the news. The tax cut which, on net, is...
The Republican tax cut has been in the news. The tax cut which, on net, is set to increase the deficit by $1.5 trillion over the next ten years is the focus of this question. A) One important thing to keep in mind is that the economy is already essentially at potential with unemployment at 4.1%. Assume that the economy is in long run equilibrium before the tax cut. What will happen to the US economy in the short run?...