With aspartate transaminase a proton tranfer from the alpha C of the amino acid was identified as a rate determining step in the overall reaction. Explain the term "rate determining step". Then briefly tell how it was possible to confirm that this prediction was true.
Rate determining step:
Generally chemical reactions occur in more than one step or in multiple steps.In the multiple steps of reaction one of the step is responsible for the rate of a chemical reaction, that step is called as rate determining step.
Definition: The slowest step in the chemical reaction which determines the rate of a chemical reaction is called as rate determining step.
Generally fast steps in a chemical reaction are highly impossible to measure the rate. so, slow step is the rate deteriming step.
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