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Determining Amino Acid Sequence • A nonapeptide was determined to have the following amino acid composition:...

Determining Amino Acid Sequence

• A nonapeptide was determined to have the following amino acid composition: – (Lys)2 Gly Leu Ala Met Phe Thr Glu

• When treated with trypsin, a tripeptide, a pentapeptide and free threonine were obtained. The tripeptides N-term was Leu, and the pentapeptides N-term was Glu 40 Determining Amino Acid Sequence • When treated with chymotrypsin, a heptapeptide and a dipeptide were obtained. The heptapeptides N-term was Leu, and the dipeptides N-term was Lys.

• When treated with CNBr, a pentapeptide and a tetrapeptide were obtained. The pentapeptides N-term was Leu, and the tetrapeptides N-term was Gly.

• What is the sequence?

please explain step by step as to how you got the answer, as if you were explaining to a person with no biology background

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