Question

The normal (wildtype) size of the brat gene is 1500 base pairs (bp). In the mutant variant you identified, there is a deletion of 500 bps in the last exon of the gene. How would you recognize the two versions based on size? List what fragment sizes you would expect to see on a gel.

Answer 1

To recognise these two different versions, we will simply use PCR followed by gel electrophoresis.

The wild-type version is 1500 base pair long and the mutant is having a deletion of 500 base pairs so it will be 100 base pairs long.

We will isolate both of these versions of the gene from the the genome of the given individual. Then using specific primers, we will amplify the to gene. We will separate these two genes using gel electrophoresis.

On the gel, we will see a band of 1500 base pairs for the wild type and a band of 1000 base pairs for the mutant.

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