Question

After you clipped the DNA, you have to multiply the DNA so you can have many...

After you clipped the DNA, you have to multiply the DNA so you can have many copies of it to test.  What is the name of this process and go through and describe the 3 steps of multiplying out the DNA

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

PCR, Polymerase chain reaction is the process by which DNA is amplified.

There are three steps in PCR, first is denaturation

Denaturation: double-stranded DNA is separated to two single-stranded DNA molecules by giving high temperature, the temperature used here is 95 to 98 degrees Celcius.

Annealing: during this stage, primer binds to specific site on the Single-stranded DNA using the rules of base pairing, the temperature of annealing depends on the primer sequence. Usually temperature used for anealing is in the range of 48 to 60 degree celcius.

Extension:During this stage DNA polymerase adds new nucleotides to the 3` end of the primer complementary to the template strand, temperature used for extension is 72 degree celcius.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
1. After re-do your culture, you now have to amplify your report gene so that you...
1. After re-do your culture, you now have to amplify your report gene so that you have enough DNA to continue your experiment. You decided to alter the concentration of template DNA strand as a testing condition. After PCR, you decided to run a gel electrophoresis to see the results of your testing condition. You loaded your ladder and enough DNA to give you bands that are bright enough to visualize. However, after you run the gel and take it...
You are attempting to clone the human insulin gene into E.Coli so that you can have...
You are attempting to clone the human insulin gene into E.Coli so that you can have access to unlimited human insulin for medical use. Insulin is produced in pacreas, but you have human skin cells to work with. 4.) So, you find the gene. Now comes the fun part, this gene must have certain features engineered into it or out of it. For you to accomplish what you need (hint it has to function in a prokaryopte now) Outline these...
Not all systems will have convenient coefficients, and you may need to multiply one or both...
Not all systems will have convenient coefficients, and you may need to multiply one or both equations by a factor that will produce coefficients of the same variable that are additive inverses, or opposites. 5. Consider solving the following system with the addition method. 3x + 2y = 5 - 2x + 5y = - 16   a. Identify which variable you wish to eliminate. Multiply the appropriate equation by the appropriate factor so that the coefficients of your chosen variable...
You are attempting to clone the human insulin gene into E.Coli so that you can have...
You are attempting to clone the human insulin gene into E.Coli so that you can have access to unlimited human insulin for medical use. Insulin is produced in pacreas, but you have human skin cells to work with. 2.) You have constructed a DNA probe, using the amino acid sequence of insulin (a short piece of DNA carrying a label, such as radioactivity, which can hybridize with your target DNA) to find your target gene. Explain whether your probe is...
Much can be learned from studying an organism’s DNA. The first step to doing this is...
Much can be learned from studying an organism’s DNA. The first step to doing this is extracting DNA from cells. In this experiment, you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder (2) 100 mL Beakers 15 cm Cheesecloth 1 Resealable Bag 1 Rubber Band (Large. Contains latex; please wear gloves when handling if you have a latex allergy). Standing Test Tube Wooden Stir Stick *Fresh, Soft Fruit (e.g., Grapes, Strawberries, Banana, etc.) *Scissors...
You would like to have enough money saved to receive $200,000 per year after retirement so...
You would like to have enough money saved to receive $200,000 per year after retirement so that you and your family can lead a good life for 30 years (from age 65 to 95). You will make your first withdraw of $200,000 at the end of year when you are 65. If you will be 35 years old when you graduate and plan on making savings contributions at the end of your first year out of school, how much would...
DNA Repair 1. Most mutations are deleterious. However, in the right environment, mutations can be advantageous....
DNA Repair 1. Most mutations are deleterious. However, in the right environment, mutations can be advantageous. Describe an example where this is the case. 2. Define mutation. 3. What are the three types of mutations that can result from modifications to the nucleotides of the DNA? 4. How are mutated nucleotides repaired? 5. Describe the process of DNA mismatch repair. 6. Why use the old strand of DNA to correct errors during DNA mismatch repair as opposed to the new...
Google has offered a $1 million prize to the lab who creates the first sequence map...
Google has offered a $1 million prize to the lab who creates the first sequence map of a dinosaur genome. We think we can do it, as we happened to get our hands on a preserved piece of dinosaur skin and were able to extract some DNA. But our lab computer guy accidentally applied his randomization algorithm to our protocol. So now the steps are all out of order. Oops. Can you put the following steps back in the correct...
You have made a mouse genomic DNA library by cutting mouse DNA with BamHI, ligating the...
You have made a mouse genomic DNA library by cutting mouse DNA with BamHI, ligating the mouse DNA fragments into the vector pBR322 at the BamHI site and then transforming the plasmids into E. coli. The pBR322 plasmid is 4.3 kb long and has a single site for the restriction enzyme BamHI. You select another bacterial colony and extract plasmid DNA. Assume that you have a 2 kb cloned fragment in this plasmid. If you digest the plasmid with BamHI,...
4. In multicellular organisms, cells have to be able to communicate with other cells so they...
4. In multicellular organisms, cells have to be able to communicate with other cells so they can work together to coordinate their activities for the benefit of the entire organism. One type of cell-cell communication involves the release of chemical signals by one cell that travel to another cell where they cause a change in the second cell (called a target cell). a. Describe the basic steps involved in the response of a target cell to a chemical signal produced...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT