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After you clipped the DNA, you have to multiply the DNA so you can have many...

After you clipped the DNA, you have to multiply the DNA so you can have many copies of it to test.  What is the name of this process and go through and describe the 3 steps of multiplying out the DNA

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Answer #1

PCR, Polymerase chain reaction is the process by which DNA is amplified.

There are three steps in PCR, first is denaturation

Denaturation: double-stranded DNA is separated to two single-stranded DNA molecules by giving high temperature, the temperature used here is 95 to 98 degrees Celcius.

Annealing: during this stage, primer binds to specific site on the Single-stranded DNA using the rules of base pairing, the temperature of annealing depends on the primer sequence. Usually temperature used for anealing is in the range of 48 to 60 degree celcius.

Extension:During this stage DNA polymerase adds new nucleotides to the 3` end of the primer complementary to the template strand, temperature used for extension is 72 degree celcius.

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