Question

You are attempting to clone the human insulin gene into E.Coli so that you can have...

You are attempting to clone the human insulin gene into E.Coli so that you can have access to unlimited human insulin for medical use. Insulin is produced in pacreas, but you have human skin cells to work with.

4.) So, you find the gene. Now comes the fun part, this gene must have certain features engineered into it or out of it. For you to accomplish what you need (hint it has to function in a prokaryopte now) Outline these features and briefly describe them.

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

Expression of eukaryotic gene is different from the prokaryotic gene. Difference comes in the forms of introns and post translational modifications. Eukaryotic gene contain introns, which have to be removed from the premature mRNA to make it mature mRNA in the post translational modifications.

This problem is solved by making a cDNA (complementary DNA) from the fully functional mRNA of eukaryotic gene.This cDNA is introduced in the prokaryotic genome to obtain desired amount of protein.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
You are attempting to clone the human insulin gene into E.Coli so that you can have...
You are attempting to clone the human insulin gene into E.Coli so that you can have access to unlimited human insulin for medical use. Insulin is produced in pacreas, but you have human skin cells to work with. 2.) You have constructed a DNA probe, using the amino acid sequence of insulin (a short piece of DNA carrying a label, such as radioactivity, which can hybridize with your target DNA) to find your target gene. Explain whether your probe is...
1) You have cloned the human Insulin (Ins) gene into the pDrive plasmid, and transformed bacteria...
1) You have cloned the human Insulin (Ins) gene into the pDrive plasmid, and transformed bacteria using (0.25 ug) of this pDrive/Ins (as per the protocol described in this lab). Using the information below, calculate the number of molecules of Insulin that have been cloned into your entire culture after 4 hours of growth after incubation. a. The transformation efficiency of this reaction is 105 colonies/ µg intact pDrive/Ins plasmid. b. pDrive plasmid has a copy number of 100 molecules...
Part III – Something's Not Right “It’s good to have you home, honey. I missed you....
Part III – Something's Not Right “It’s good to have you home, honey. I missed you. How was the flight?” Stacey had come to the airport to pick Frank up and she leaned over to kiss him as he climbed into the car with his luggage. “How were the meetings? You look tired,” she added. “The past week was intense and I am exhausted. I thought I would manage some R & R during the trip, but no such luck....
Question: summarize what you have read and how you believe (based on what we've studied so...
Question: summarize what you have read and how you believe (based on what we've studied so far) that topic should be approached from a law and policy perspective. Introduction The U.S.-Cuba relationship has been plagued by distrust and antagonism since 1959, the year Fidel Castro overthrew a U.S.-backed regime in Havana and established a socialist state allied with the Soviet Union. During the half century that followed, successive U.S. administrations pursued policies intended to isolate the island country economically and...
Assignment: What are the main arguments in the article? Please answer within 5 hours. It is...
Assignment: What are the main arguments in the article? Please answer within 5 hours. It is extremely urgent!!!!!!!!!!!!!!!!!!!!!!!! --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- BIOETHICS. Bioethics as a field is relatively new, emerging only in the late 1960s, though many of the questions it addresses are as old as medicine itself. When Hippocrates wrote his now famous dictum Primum non nocere (First, do no harm), he was grappling with one of the core issues still facing human medicine, namely, the role and duty of the...
What topics are covered in the following article? Please answer within 5 hours. It is extremely...
What topics are covered in the following article? Please answer within 5 hours. It is extremely urgent!!!!!!!!!!!!!!!!!!!!!!!! --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- BIOETHICS. Bioethics as a field is relatively new, emerging only in the late 1960s, though many of the questions it addresses are as old as medicine itself. When Hippocrates wrote his now famous dictum Primum non nocere (First, do no harm), he was grappling with one of the core issues still facing human medicine, namely, the role and duty of the physician....
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old...
READ THE CASE STUDY AND ANSWER THE FOLLOWING QUESTIONS 2nd CASE: An Unexplained Death A 65-year-old man of Scandinavian descent was rushed to the Emergency Room of your local hospital after a family member discovered him unconscious in his home. The woman who dialed “911” told the dispatcher that the man, her brother, was the local librarian of the past 10 years and had no spouse or children. She reported that they had spoken the day before, and he had...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT