Question

dische diphenylamine will be used during the experiment that tests for which macromolecule? a) Carbohydrates b)...

dische diphenylamine will be used during the experiment that tests for which macromolecule?
a) Carbohydrates
b) Lipids
c) Proteins
d) Nuclei Acids
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Answer #1

Dische diphenylamine test is used for the detection of DNA in a sample. DNA has deoxyribose sugar while RNA has ribose sugar. The 2-deoxyprntose sugar present in DNA reacts with the dische reagent to form a blue colour. The deoxyriboase sugar is first converted to w-hydroxylaevulinic aldehyde, when DNA is heated in acid. The w-hydroxylaevulinic aldehyde under acidic conditions will react with diphenylamine to form a blue colored complex. Nitrates are responsible for development o blue colour. The concentration of the DNA is directly proportional to the blue colored complex, which is measured at 595 nm. Ribose sugars do not react with Dische reagent.

Option is d) Nucleic acid.

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