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If I needed to determine if a pneumonia was bacterial, fungal, or viral. Would it be...

If I needed to determine if a pneumonia was bacterial, fungal, or viral. Would it be correct to hypothesize that:If the microbe causing the patient’s pneumonia is a bacteria, then a gram stain of the specimen slide will reveal an organism with a cell wall, lacking a nucleus?

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No, this is not true. If the microbe causing the patient’s pneumonia is a bactera then it should have a nucleus. Streptococcus pneumoniae is the most common bacterial cause of pneumonia, and it is a gram positive bacteria. So, their cell wall will be clearly visible with purple color, and they have the nucleus. In addition, several gram negative bacteria can cause pneumonia such as Haemophilus influenzae, Klebsiella pneumoniae, but they are less frequent. Bacteria always posses nucleus, and can be clearly visible after gram staining (under microscope).

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