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Carry out your own DNA extraction experiment from plant cells   Do not forget to document everything...

Carry out your own DNA extraction experiment from plant cells  

Do not forget to document everything !

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Answer #1

Protocol of the experiment

1. DNA could be isolated from any tissue of the plant cell but most preferable is young leaf tissue

2. Then the tissue is kept in a tube and filled with frozen nitrogen. Such a cool nitrogen mechanically break the cell wall.

3. Pulverise the cell in tube.

4. In the tissue, add the buffer, salt and detergent.

5. Buffer and salt stabilize the DNA whereas detergent break the plasma membrane .

6. Heat the cell now and incubated at 60 degree to separate DNA from other cellular component.

7. Chloroform is added to the tube so that it wash away the unwanted components from the DNA present in DNA.

8. Then centrifuge the DNA

9. DNA is separated with the help of centrifugation.

10 .Ethanol is added so that DNA is precipatated from the sample .

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