Question

Using the appropriate equations, show why fluorescence can be more sensitive than absorption?

Answer 1

Among the two analytical unstrumental techniques, the sensitivity of fluorescence technique is almost thousand times sensitive than absobtion technique.In absobtion spectrophotometry, quantitative estimation of sample can done by taking the measurement of absorbtion. eqation is beers laberts law A= Cl

where in case of fluorescence , when a molecule absorbs energy in the form of light and as soon as possible it releases energy again in the form of emitting light . charecteristic wavelength of that molecule emits is taken into consideration.emitted light read at the right angles to the exiting light and the backround signal can be minimized and this technique has larger range of linearity.hence fluorenscence is more sensitive than absorbtion.