Question

Cite the purpose of each of the following reagents in the gram stain procedure: Crystal violet...

  1. Cite the purpose of each of the following reagents in the gram stain procedure:
    1. Crystal violet --
    1. Gram’s iodine –
    1. 96% ethyl alcohol –
    1. Safranin --
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Answer #1

Bacter

Crystal violet. is used to to stain bacterial cell wall.

Iodine is subsequently added as a mordant to form the crystal violet-iodine complex so that the dye cannot be removed easily.

ethyl alcohol can be used as the decolorizing agent. The alcohol dissolves lipids found in the outer cell membrane of Gram-negative bacteria, allowing the crystal violet-iodine complex to leak out of the thinner peptidoglycan layer.

The safranin is also used as a counter-stain in Gram's staining. In Gram's staining, the safranin directly stains the bacteria that has been decolorized. With safranin staining, the gram-negative bacteria can be easily distinguished from gram-positive bacteria.

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