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Why were each of the following steps performed in the experiment(1 Bioprospecting for Starch-Degrading Bacteria )...

Why were each of the following steps performed in the experiment(1 Bioprospecting for Starch-Degrading Bacteria )

a.Serial dilution

b.Growth on the nutrient agar plates

c.Transfer growth to the starch agar plates

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Answer #1

Serial dilution: It is normally done if the chemical concentration is too high than the desired composition. It is used to diminish the thick culture to a more usable concentration.

Growth on the nutrient agar plates: Growth on the nutrient agar plates is carried out for isolation and purification of cultures. This is used to produce the bacterial lawns needed for the tests. When the growth appears on the agar we are able to perform our startch degrading bacterial experiments. Therefore, this is used for isolation and purification of culture ultimately.

Streak on the starch agar plates: Streak on the starch agar plates is a qualitative isolation method in order to isolate and sample individual bacterial colonies.

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