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A culture of Staphylococcus of unknown density is diluted as follows (letters represent each step): 60...

A culture of Staphylococcus of unknown density is diluted as follows (letters represent each step): 60 mL of a bacterial culture is diluted by adding 540 mL of water 1000 microliters from (A) is added to 9 mL of water Dilution (B) is used to prepare a 10-3 dilution in water 100 uL from dilution (C) is plated to nutrient agar and incubated at 37oC Following incubation, colonies are uniformly distributed on the agar, and 26 colonies are counted on one quarter of the plate. 1. Express the total CFU/mL of the original culture in scientific notation. 2. If 10 uL is plated from dilution (C), how many colonies should grow on the plate?

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