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Why is measuring the O.D and calculating the theoretical cell concentration not sufficient? Will having a...

Why is measuring the O.D and calculating the theoretical cell concentration not sufficient? Will having a cell culture with a mix of living/dead cells make a difference?

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O.D. i.e. optical density measuring and calculating the theoretical cell concentration is not sufficient because it measures both living and dead cells concentration and can't differentiate living cells from dead cells. O.D. measurement works on the principle of reflection of light and both living and dead cells will reflect light and taken into count for calculating theoretical cell concentration. So, O.D. is not sufficient for calculating theoretical viable cell concentration.

No, it will not make a difference with a cell culture having mix of both living and dead cells. O.D. measures reflection of light from cells. So, it will measure concentration of both dead and live cells.

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