Question

You want 50 fmol DNA in a 10 µL reaction. MW of DNA is 607.4 g/bp....

You want 50 fmol DNA in a 10 µL reaction. MW of DNA is 607.4 g/bp. Your DNA is 500 bp in length and at a concentration of 10 ng/µL. How many µL of your DNA should you add to the reaction?

Science   Biology   
0 0
Add a commentTranscribed image text
Next >

Homework Answers

Answer #1

The information suggests that the size of DNA is 500bp and the molecular weight is 607.4 g/bp. Thus, the weight of available DNA is 607.4*500 or 303700g or 0.31 microgram or 3.1*10-4 ng. The required concentration of DNA is 50fmol i.e. 50*10-15 mol.

Using the formula:

M1V1 = M2V2,

10 * 10-9 * V1= 50*10-15 * 10

Solving for V1, we get V1 =50*10-15 / 10-8

or V1 = 5*10-6 ul or 5 picolitre.

Thus, only 5 picolitre of this stock solution is required to be mixed in a reaction volume of 10 ul to obtain the desired concentration.

Since this small volume cannot be measured using any pipette, serial dilutions of the stock solution are first made until an requisite dilution is reached and this final dilution is used for preparing the reaction mixture.

0 0
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
You have a phenomenal day in lab and collect 35 µg of TAS2R38 PCR product in...
You have a phenomenal day in lab and collect 35 µg of TAS2R38 PCR product in 50 µl of nuclease free water. How many µl of this stock would you use to make 30 µl of 2.5 ng/µl DNA final concentration for the DNA sequencing core? Round to the nearest tenth. (Show work for partial credit.)
Concentration (µl/mL) % Mortality 0 0% 10 10% 50 50% 100 70% 500 90% 1000 100%...
Concentration (µl/mL) % Mortality 0 0% 10 10% 50 50% 100 70% 500 90% 1000 100% this table shows the mortality rate in worms population vs concentration of a certain insectiside please help me to determine the NOAEL and LOAEL on the graph and how to define them specifically and accurately
Create a protocol for a restriction digest with a total volume of 30 µl to digest...
Create a protocol for a restriction digest with a total volume of 30 µl to digest 1.0 µg of plasmid DNA to completion. You have a restriction enzyme in a standard 50% glycerol at 5 Units / µl but wish to do a standard 'overdigestion' in which you will put the maximum allowable enzyme in the digest. (Indicate how many Units will be in the digest.) You have a compatible 10X restriction buffer, and your DNA is at 0.3 µg...
You are provided with a 0.1 mM solution of proflavine (MW: 209.25 g/mol), a 6 mM...
You are provided with a 0.1 mM solution of proflavine (MW: 209.25 g/mol), a 6 mM solution of DNA, and solid NaCl (MW: 58.44 g/mol). How many μL of the DNA solution would you need to make a 10 mL of the following stock solution: 1 µM proflavine, 100 mM NaCl, and 60 µM DNA? State your answer as an integer value (no decimal point). State only the number, not the unit.
You are provided with a 0.1 mM solution of proflavine (MW: 209.25 g/mol), a 6 mM...
You are provided with a 0.1 mM solution of proflavine (MW: 209.25 g/mol), a 6 mM solution of DNA, and solid NaCl (MW: 58.44 g/mol). How many mg of NaCl would you need to make a 15 mL of the following stock solution: 1 µM proflavine, 100 mM NaCl, and 60 µM DNA? Round your answer to one decimal digit. State only the number, not the unit.
Generate three ligation reactions with 1:1, 2:1 and 3:1 molar ratios of the insert and the...
Generate three ligation reactions with 1:1, 2:1 and 3:1 molar ratios of the insert and the vector DNA. Your ligation should include all components in a 30 µL reaction. Keep the vector amount fixed at 80 ng per ligation reaction. You are provided with 10 x ligase buffer and DNA ligase (0.5 U/µL) to set up your ligations.
1. a) You have a stock tube of 50 mM MgCl2 and you need to optimize...
1. a) You have a stock tube of 50 mM MgCl2 and you need to optimize MgCl2 concentration for the Q8 SYBR Green primers. Create a table to describe how you would create a dilution series so that MgCl2 could be tested at 5 mM, 4.5 mM, 3 mM, 2.5 mM, 1 mM, 0.5 mM and 0 mM to optimize a 25 l PCR (see the table below). Your new stocks should be in a 100 μL volume. b) Once...
You are adding 2 μL of a 10X restriction buffer to a reaction that will total...
You are adding 2 μL of a 10X restriction buffer to a reaction that will total 20 μL. What is the final concentration of buffer in the reaction (in terms of “X”)? Add 4 μL of 0.1 ug/uL DNA to each reaction tube. How many ug of DNA are being digested in this reaction? When you have set up all of your reactions, bring your reactions in your ice bucket to the front of the room to add 1 μL...
You are purifying bacterial chromosomal DNA to send off for genome sequencing. The estimated genome size...
You are purifying bacterial chromosomal DNA to send off for genome sequencing. The estimated genome size of your organism is 4 Mbp and you know that the average MW of a base pair is 660 Daltons. You have isolated 1 microg of genomic DNA, how many moles of DNA are in your sample? If you resuspended the chromosomal DNA in 50 microL, what would be the final concentration of the DNA?
Your new <$1 million grant has afforded you the ability to isolate DNA from bacterial cells....
Your new <$1 million grant has afforded you the ability to isolate DNA from bacterial cells. You want to estimate how much DNA is present in your sample. You use 30 µL of sample, add 270 µL of buffer, and receive an A260 of 0.40 and A280 of 0.25. (You can use whichever method you prefer on (a) and (b), but be consistent for both. a. (2 pt) Assuming the sample was pure, about how much double-stranded DNA was present...
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT