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Generate three ligation reactions with 1:1, 2:1 and 3:1 molar ratios of the insert and the...

  1. Generate three ligation reactions with 1:1, 2:1 and 3:1 molar ratios of the insert and the vector DNA. Your ligation should include all components in a 30 µL reaction. Keep the vector amount fixed at 80 ng per ligation reaction. You are provided with 10 x ligase buffer and DNA ligase (0.5 U/µL) to set up your ligations.
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