Question

The rate of cooling to the final storage temperature can profoundly affect the rate of the frozen biological sample. Describe thecellular damage that occurs at both slow and rapid rates of cooling.

Answer 1

Cellular damage occurs at both Slow and Rapid Rates of Cooling

From this we get that cell is damage at both stages i.e slow or rapid. It means there is some optimal rate of cooling which doesnt damage the cell.

Excessively rapid cooling rates leads to intracellular ice formation i.e IIF

Slow cooling rates has been shown to accumulate with exposure to high concentration of solutes and known as "Solution Effect" Injury

Therefore there are two types of injury mechanism

1.Slow rapid- Which can be avoided /improved by increasing the cooling rate

2. Rapid -Which can be avoided/reduced by slowing the cooling rate.

The tempertaure range of both the injuries lie between -15O C to -40OC

Suffering of cells during freezing which further affects their biological functions.Cryopreservation