Question

Sort the descriptions below to indicate whether they describe ? oxidation of stearoyl-CoA, oleoyl-CoA, or both....

Sort the descriptions below to indicate whether they describe ? oxidation of stearoyl-CoA, oleoyl-CoA, or both.

8 NAD+ reduced

8 FAD reduced

? oxidation completed in 8 rounds

7 FAD reduced

Requires ?3,?2-enoyl-CoA isomerase required for completion of round 4

9 acetyl-CoA produced

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Answer #1

The descriptions indicate oxidation of both stearoyl-CoA and oleoyl-CoA. Both are 18 carbon faaty acids. Stearoyl-CoA is saturated fatty acid while oleoyl-CoA is monounsaturated fatty acids.

Stearoyl-CoA oxidation: Since stearoyl-CoA is 18 carbon compunds, 9 acetyl-CoA are produced in 8 rounds of oxidation and therefore 8 NADH and 8 FADH2 are formed.

Oleoyl-CoA oxidation: The oxidation of oleoyl-CoA is little bit different because it is an unsaturated fatty acid. Here formation of a trans-?2 bond is a problem because of presence of double bond. This situation is handled by an extra enzyme enoyl-CoA isomerase which convert the double bond to a trans-?2 bond in round 4. Here one less molecule of FADH2 produce because of this additional step.

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