Question

Indicate whether the statement is true for Sanger sequencing, PCR, both, or neither:

1.Requires dNTPs

2. Requires a palindromic DNA sequence

3. Requires ddNTPs

4. Requires a thermostable DNA polymerase (e.g. , Taq polymerase)

5. Uses a pair of primers

6. Amplifies DNA at an exponential rate

7. Requires DNA ligase

8. DNA is elongated by adding a dNTP to the 3' hydroxyl

9.Requires at least some knowledge of the target DNA to create a primer

Answer 1

1. Requires dNTPs - Both
2. Requires a palindromic DNA sequence - Neither
3. Requires ddNTPs - Sanger Sequencing, as the ddNTP acts as a chain-terminator
4. Requires a thermostable DNA polymerase (e.g. , Taq polymerase) - Both
5. Uses a pair of primers - Both
6. Amplifies DNA at an exponential rate - PCR, hence Polymerase Chain Reactor
7. Requires DNA ligase - Neither
8. DNA is elongated by adding a dNTP to the 3' hydroxyl - Both
9.Requires at least some knowledge of the target DNA to create a primer - Both