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What is/would be the positive control in an Ames Test experiment?

What is/would be the positive control in an Ames Test experiment?
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The test is named to honor Bruce Ames, he was the first who identified the utility of assay for the detection of mutations in 1974 (Ames, 1974).It is an assay which basically done to measures bacterial colony formation . The experiment comprises histidine gene for the mutation of strains of Salmonella typhimurium there is histidine used for the growth of these strains. Auxotrophic bacteria actually treated with a test chemical or vehicle or positive control on an agar plate consisting of growth media that lacks histidine. Histidine used for bacterial growth Thus, bacteria those have a capability to reverted back to histidine can grow and formation of colonies.

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