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What is positive control and negative control in a biological assay? Let’s assume you want to...

What is positive control and negative control in a biological assay? Let’s assume you want to analyze the expression of the bcr-abl chimeric gene in a patient diagnosed with Chronic Myeloid Leukemia by qRT-PCR. What will your negative and positive controls be?

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Ans: A negative control is a control group in an experiment that uses a treatment that isn't expected to produce results. A positive control is a control group in an experiment that uses a treatment that is known to produce results.

In the analysis of the expression of the BCR-ABL chimeric gene in a patient diagnosed with Chronic Myeloid Leukemia by q-PCR, cDNA synthesized from K562 cells (B3A2) and KCL22 for B2A2 cells type and ALL/MIK for p190 e1a2 were used as positive controls and sterile water was used as negative controls. In this study, we used ABL gene as a control gene.

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